Journal: Molecular cell

Article Title: Lineage-restricted regulation of SCD and fatty acid saturation by MITF controls melanoma phenotypic plasticity

doi: 10.1016/j.molcel.2019.10.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: SKmel28 Human melanoma cell line (male) , Obtained from ATCC , ( Fogh et al., 1977 ).

Techniques: Isolation, Virus, Subcloning, Bacteria, Recombinant, Multiplex sample analysis, Labeling, Reverse Transcription, RNA Extraction, Reporter Assay, Bicinchoninic Acid Protein Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control, Plasmid Preparation, Expressing, Construct, Luciferase, Software

Journal: International Journal of Oncology

Article Title: Bromodomain inhibitor jq1 induces cell cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway

doi: 10.3892/ijo.2019.4863

Figure Lengend Snippet: JQ1 induces cycle arrest and apoptosis of glioma stem cells through the VEGF/PI3K/AKT signaling pathway. (A) The mRNA expression of genes related growth factors in CSC2078 expressed as fold change. (B and C) Western blotting analysis of VEGF, MMP9 and MMP2 expression in CSC2078 treated with JQ1 (error bars represent standard error of the mean). (D and E) CSC2078 cells were treated with JQ1 and or linifanib for 24 h. JQ1=100 nM, linifanib=5 µ M. The cell cycle was analyzed by flow cytometry. Quantitation of the cell cycle. Data are representative of three independent experiments (error bars represent standard error of the mean). (F and G) CSC2078 cells were treated with JQ1 and or linifanib for 24 h. JQ1=100 nM, linifanib=5 µ M. Effects of CSC2078 apoptosis were detected by flow cytometry. Quantitation of the apoptosis rate of CSC2078. Data are representative of three independent experiments. (Error bars represent standard error of the mean). (H and I) Western blotting analysis of p-VEGFR2, VEGFR2, PI3K, Akt, p-Akt (Ser473), CyclinB1, Bcl-2 and Bax in control, JQ1 and/or linifanib treated groups. JQ1=100 nM, linifanib=5 µ M. Densitometry was performed and fold change of protein expression was presented (error bars represent standard error of the mean). * P<0.05, ** P<0.01 vs. Con. Bax, Bcl2-associated X protein; Con, control; J/C, JQ1/Con; MMP, matrix metalloproteinase; p, phosphorylated; VEGFR2, vascular endothelial growth factor; VEGFR2, receptor 2.

Article Snippet: The slides were incubated with primary antibodies overnight at 4°C, against c-Myc (cat. no. 10828-1-AP, 1:100), BAX (cat. no. 50599-2-lg, 1:100), BCL2 (cat. no. 12789-1-AP, 1:100), MMP2 (cat. no. 10373-2-AP, 1:100) and MMP9 (cat. no. 10375-2-AP, 1:100) from ProteinTech Group, In.

Techniques: Expressing, Western Blot, Flow Cytometry, Quantitation Assay, Control